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human full length angptl4 protein  (R&D Systems)


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    R&D Systems human full length angptl4 protein
    Human Full Length Angptl4 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human full length angptl4 protein/product/R&D Systems
    Average 94 stars, based on 3 article reviews
    human full length angptl4 protein - by Bioz Stars, 2026-06
    94/100 stars

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    human full length angptl4 protein  (R&D Systems)
    94
    R&D Systems human full length angptl4 protein
    Human Full Length Angptl4 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human full length angptl4 protein/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    human full length angptl4 protein - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    recombinant full length murine angptl4  (R&D Systems)
    94
    R&D Systems recombinant full length murine angptl4
    Figure 1. Temporal expression of <t>Angptl4</t> mRNA in the femoral fracture callus. (A) Expression of select genes in the fracture callus quantified using the mouse angiogenic growth factors and angiogenesis inhibitors RT2Profiler PCR array (SA Biosciences). Results were normalized to the loading control Actb to yield DCt. Bars represent mean SEM, n ¼ 3. (B) Expression of Angptl4 mRNA during fracture healing or in sham operated mice. Results are expressed as mean 2DDCt SEM, with expression normalized to Actb transcript level and contralateral limb, n ¼ 3. *p < 0.05. ** p < 0.005.
    Recombinant Full Length Murine Angptl4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant full length murine angptl4/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    recombinant full length murine angptl4 - by Bioz Stars, 2026-06
    94/100 stars
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    Figure 1. Temporal expression of Angptl4 mRNA in the femoral fracture callus. (A) Expression of select genes in the fracture callus quantified using the mouse angiogenic growth factors and angiogenesis inhibitors RT2Profiler PCR array (SA Biosciences). Results were normalized to the loading control Actb to yield DCt. Bars represent mean SEM, n ¼ 3. (B) Expression of Angptl4 mRNA during fracture healing or in sham operated mice. Results are expressed as mean 2DDCt SEM, with expression normalized to Actb transcript level and contralateral limb, n ¼ 3. *p < 0.05. ** p < 0.005.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 1. Temporal expression of Angptl4 mRNA in the femoral fracture callus. (A) Expression of select genes in the fracture callus quantified using the mouse angiogenic growth factors and angiogenesis inhibitors RT2Profiler PCR array (SA Biosciences). Results were normalized to the loading control Actb to yield DCt. Bars represent mean SEM, n ¼ 3. (B) Expression of Angptl4 mRNA during fracture healing or in sham operated mice. Results are expressed as mean 2DDCt SEM, with expression normalized to Actb transcript level and contralateral limb, n ¼ 3. *p < 0.05. ** p < 0.005.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Expressing, Control

    Figure 2. Detection of Angptl4 mRNA during fracture healing. Serial sections were taken through the mid-diaphysis (control, unfractured bone day 0) and the mid-callus at days 2 and 7. Sections were stained with H&E to demonstrate morphology (H&E/Alcian Blue), underwent in situ hybridization staining for Angplt4 mRNA (Angplt4 mRNA) or represent no probe controls (Control).

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 2. Detection of Angptl4 mRNA during fracture healing. Serial sections were taken through the mid-diaphysis (control, unfractured bone day 0) and the mid-callus at days 2 and 7. Sections were stained with H&E to demonstrate morphology (H&E/Alcian Blue), underwent in situ hybridization staining for Angplt4 mRNA (Angplt4 mRNA) or represent no probe controls (Control).

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Control, Staining, In Situ Hybridization

    Figure 3. Detection of Angptl4 mRNA in the callus 7 days post femoral fracture. Serial sections were taken through the mid-callus to show callus cartilage (A–C) and new bone formation (D–F) and mineralizing osteoblasts at higher magnification (G–I). White x’s mark the condensing mesenchyme, asterisks mark the original cortical bone, and brackets designate the callus cartilage (A) or areas of new bone formation (D). Panels A, D, and G were stained with H&E/Alcian Blue, panels B, E, and H demonstrate in situ hybridization staining for Angplt4 mRNA and panels C, F, and I are no probe controls.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 3. Detection of Angptl4 mRNA in the callus 7 days post femoral fracture. Serial sections were taken through the mid-callus to show callus cartilage (A–C) and new bone formation (D–F) and mineralizing osteoblasts at higher magnification (G–I). White x’s mark the condensing mesenchyme, asterisks mark the original cortical bone, and brackets designate the callus cartilage (A) or areas of new bone formation (D). Panels A, D, and G were stained with H&E/Alcian Blue, panels B, E, and H demonstrate in situ hybridization staining for Angplt4 mRNA and panels C, F, and I are no probe controls.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Staining, In Situ Hybridization

    Figure 5. Hypoxic Regulation of Angptl4 mRNA in MC3T3-E1 osteoblastic cells. Expression of Vegfa, (A) and Angptl4 mRNA (B) at 6, 24, and 48 h after start of treatment with 21% oxygen, 1% oxygen or 100 mg/ml desferrioxamine (DFO). Bars represent mean 2DCt SEM, with expression normalized to Tuba1a, n ¼ 3. *p < 0.05.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 5. Hypoxic Regulation of Angptl4 mRNA in MC3T3-E1 osteoblastic cells. Expression of Vegfa, (A) and Angptl4 mRNA (B) at 6, 24, and 48 h after start of treatment with 21% oxygen, 1% oxygen or 100 mg/ml desferrioxamine (DFO). Bars represent mean 2DCt SEM, with expression normalized to Tuba1a, n ¼ 3. *p < 0.05.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Expressing

    Figure 4. Detection of Angptl4 mRNA in normal murine femora. Serial sections were taken of articular cartilage (A–C), and growth plate (D–F) at 20 magnification. Brackets represent articular cartilage (A and B) and growth plate (D and E). Panels A and D were stained with H&E/Alcian Blue to demonstrate normal histological appearance of the tissues, panels B and E demonstrate in situ hybridization staining for Angplt4 mRNA and panels C and F are no probe controls.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 4. Detection of Angptl4 mRNA in normal murine femora. Serial sections were taken of articular cartilage (A–C), and growth plate (D–F) at 20 magnification. Brackets represent articular cartilage (A and B) and growth plate (D and E). Panels A and D were stained with H&E/Alcian Blue to demonstrate normal histological appearance of the tissues, panels B and E demonstrate in situ hybridization staining for Angplt4 mRNA and panels C and F are no probe controls.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Staining, In Situ Hybridization

    Figure 7. Effects of exogenous ANGPTL4 on expression of Vegfa, and genes related to osteoblastic differentiation. Expression of Vegfa (A), Runx2, (B) Spp1 (C), Bglap(D), and Alp(E) mRNA at 7, 14, and 21 days in osteogenic differentiation media supplemented with 100 or 250 ng/ml Angptl4. Bars represent mean 2DCt SEM, with expression normalized to Tuba1a, n ¼ 3. *p < 0.05. Undifferentiated represents cultures maintained in standard growth media.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 7. Effects of exogenous ANGPTL4 on expression of Vegfa, and genes related to osteoblastic differentiation. Expression of Vegfa (A), Runx2, (B) Spp1 (C), Bglap(D), and Alp(E) mRNA at 7, 14, and 21 days in osteogenic differentiation media supplemented with 100 or 250 ng/ml Angptl4. Bars represent mean 2DCt SEM, with expression normalized to Tuba1a, n ¼ 3. *p < 0.05. Undifferentiated represents cultures maintained in standard growth media.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Expressing

    Figure 6. Angptl4 mRNA expression during osteogenic differ- entiation. Angptl4 mRNA expression at 7, 14, and 21 days in osteogenic differentiation media. Bars represent mean 2DCt SEM, with expression normalized to Tuba1a, n ¼ 4. *p < 0.05. Undifferentiated represents cultures maintained in standard growth media.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 6. Angptl4 mRNA expression during osteogenic differ- entiation. Angptl4 mRNA expression at 7, 14, and 21 days in osteogenic differentiation media. Bars represent mean 2DCt SEM, with expression normalized to Tuba1a, n ¼ 4. *p < 0.05. Undifferentiated represents cultures maintained in standard growth media.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Expressing

    Figure 8. Effects of exogenous ANGPTL4 on osteoblastic mineral production. Quantitation of (A) hydroxyapatite by Osteo- Image and (B) calcium by Alizarin red at various timepoints after culture in osteogenic differentiation media supplemented with 100 or 250 ng/ml Angptl4. Bars represent mean SEM, n¼4. *p < 0.05 and ****p < 0.0001. (C) Representative Alizarin red staining at 0, 7, 14, and 21 days in osteogenic differentiation media supplemented with 100 or 250 ng/ml Angptl4. Undifferen- tiated represents cultures maintained in standard growth media.

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Expression of angiopoietin-like protein 4 at the fracture site: Regulation by hypoxia and osteoblastic differentiation.

    doi: 10.1002/jor.22898

    Figure Lengend Snippet: Figure 8. Effects of exogenous ANGPTL4 on osteoblastic mineral production. Quantitation of (A) hydroxyapatite by Osteo- Image and (B) calcium by Alizarin red at various timepoints after culture in osteogenic differentiation media supplemented with 100 or 250 ng/ml Angptl4. Bars represent mean SEM, n¼4. *p < 0.05 and ****p < 0.0001. (C) Representative Alizarin red staining at 0, 7, 14, and 21 days in osteogenic differentiation media supplemented with 100 or 250 ng/ml Angptl4. Undifferen- tiated represents cultures maintained in standard growth media.

    Article Snippet: To examine the effects of exogenous ANGPTL4 on osteogenesis, standard culture media was replaced 24h after plating with differentiation media supplemented with 0, 100, or 250ng/ml recombinant full length murine ANGPTL4 (R&D systems, Minneapolis, MN).

    Techniques: Quantitation Assay, Staining